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KMID : 0359319950350010095
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Korean Journal of Veterinary Research
1995 Volume.35 No. 1 p.95 ~ p.105
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Analysis of human HoxA gene control region and its effects on anterior - posterior axial pattern formation using transgenic mouse embryo
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김명희/Kim, Myoung Hee
박종훈/이철상/이경광/민원기/이영원/전무형/Park, Jong Hoon/Lee, Chul Sang/Lee, Kyung Kwang/Min, Won Gi/Lee, Young Won/Jun, Moo Hyung
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Abstract
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The human homolog of position specific element of mouse Hoxa-7 was studied using transgene. It contains a 1.1 kb human DNA (HCR) a homolog to the intergenic region between Hoxa-7 and -9, which directs the position specific expression of Hoxa-7-, tk promoter, LacZ (β-galactosidase) gene as a reporter, and polyadenylation signal of SV40 large T antigen. It was injected into the mice embryos, and the resulting transgenic embryos were analysed through PCR as well as genomic Southern blotting with placenta DNA. Out of 20 embryos analysed, two were transgenic. Among them, one transgenic embryo expressed transgene when stained with X-gal. The expression pattern was in analogy to that of the mouse Hoxa-7, showing spatially restricted expression pattern. Since the expression of β-galactosidase is regulated by the upstream human HCR sequence, it implies that the HCR is the plausible position specific regulatory element of human
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